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room temperature (Thermo Fisher)

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Thermo Fisher room temperature
Room Temperature, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/room+temperature/Paraformaldehyde%2C+16%25+w%2Fv+aq%2E+soln%2E%2C+methanol+free/pm42320481-447-4-9
Average 98 stars, based on 1 article reviews

room temperature - by Bioz Stars, 2026-09

98/100 stars

Images

Staining:

Article Title: 3D-Printed Biphasic PCL/β-TCP Scaffold with Spatially Confined GelMA/CS Hydrogel for Coordinated Osteochondral Regeneration
Article Snippet: .. Samples were fixed with 4% paraformaldehyde (PFA) in PBS for 15 min at room temperature, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 1% bovine serum albumin (BSA) in PBS for 60 min. F-actin was stained with FITC-conjugated phalloidin (1:200 dilution; Thermo Fisher Scientific) for 60 min at room temperature in the dark, and nuclei were counterstained with DAPI (1 μg mL−1) for 5 min. Fluorescence images were acquired using a confocal laser scanning microscope (LSM 710, Zeiss, Germany). ..

Fluorescence:

Laser-Scanning Microscopy:

Blocking Assay:

Article Title: PDIA6-SCD1 Axis Rewires Lipid Metabolism to Drive Gastric Cancer Progression.
Article Snippet: .. Cells were fixed in 4% paraformaldehyde for 15 min at room temperature and permeabilized with 0.5% Triton X-100 for 20 min. After blocking with 5% bovine serum albumin (BSA) for 1 h, cells Advanced Science, 2026 tive C om m ons L icense w P 2 f f D c m w 5 T c s I t p s c o 5 s f a e T e t p r b b m a t m f 5 A C u t 1 c 5 1 p D K a e C A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.75923 by U niversidade Federal D a Paraiba, W iley O nline L ibrary on [05/06/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C rea ere incubated overnight at 4◦C with primary antibodies against DIA6 (Proteintech, 18233-1-AP; 1:1000) and SCD1 (Proteintech, 8678-1-AP; 1:1000).